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- Title
Somatostatin activates particulate guanylate cyclase in cultured rat mesangial cells.
- Authors
García-Escribano, Carmen; Luisa Díez-Marqués, M.; Medina-Alonso, Jesús; Rodríguez-Puyol, Manuel; Rodríguez-Pyoul, Diego
- Abstract
Although the ability of somatostaiin (ST) to relax cultured rat mesangial cells has recently been described, the intimate cellular mechanisms responsible for this effect have not been adequately clarified. The present experiments were designed to test the hypothesis that cyclic GMP (cGMP) could be involved in the genesis of this relaxation. ST increased cGMP synthesis by cultured rat mesangial cells, in basal conditions and in the presence of isobulylmethylxanthine or zaprinast. This effect has dose-dependent, with a threshold value of about 1 nM and a maximal response at ST concentrations between 0.1 and 1 μM. This increased cGMP synthesis was dependent on the stimulation by ST of a particulate guanylate cyclase, as the synthesis of cGMP by a particulate memebrane fraction obtained from the cells increased in the presence of ST. When the cGMP-specific phosphodiesterase of mesangial cells was blocked with zaprinast, the ST-dependent relaxation, assessed both by morphological and biochemical criteria, significantly increased with respect to the experiments performed without zaprinast. These results support a role for cGMP in the ST-dependent relaxation of cultured rat mesangial cells. The increased cGMP synthesis appears to be the consequence of the activation of some form of particulate guanylate cyclase.
- Subjects
SOMATOSTATIN; GUANYLATE cyclase; CELL culture; PEPTIDE hormones; CYCLIC guanylic acid
- Publication
Kidney International, 1994, Vol 46, Issue 6, p1611
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1111/1523-1755.ep14899453