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- Title
Hemodialysis and red cell cation transport in uremia: Role of membrane free fatty acids.
- Authors
Kelly, Ralph A.; Canessa, Mitzy L.; Steinman, Theodore I.; Mitch, William E.
- Abstract
Active and facilitated cation transport in erythrocytes of uremic patients may be improved acutely by hemodialysis, although the mechanisms remain unknown. As nonesterified fatty acids (NEFA) can affect Na+ pump activity in vitro, changes in plasma and red cell membrane NBFA content following a single hemodialysis procedure were examined and compared with acute changes in erythrocyte cation flux rates in 34 hemodialysis patients. In nonsodium-loaded cells, small changes in Na+ pump flux with dialysis did correlate with changes in intracellular Na+ content (r = 0.59; N = 17; P < 0.01). On average, neither maximal Na+ pump activity nor Na+/Li+ counter-transport flux improved with dialysis, but Na+/K+/Cl- cotransport rates rose 25% post-dialysis (P < 0.02), Plasma NEFA levels rose 87% following hemodialysis but erythrocyte membrane NEFA content declined by 23% (P < 0.001). Importantly, 24 of the 34 subjects studied had a decrease in erythrocyte membrane NEFA content of greater than 10%, and in these patients, the fall in membrane NEFA correlated with an increase in ouabain-sensitive Na+ efflux (r = 0.564; P < 0.01). The effects of hemodialysis on both erythrocyte NEFA content and Na+ pump flux could he reproduced by incubating pre-dialysis cells in fatty acid-free albumin. We conclude that acute changes in membrane NEFA may modulate active cation transport in uremic erythrocytes.
- Subjects
HEMODIALYSIS; ERYTHROCYTES; CATIONS; FATTY acids; DIALYSIS (Chemistry)
- Publication
Kidney International, 1989, Vol 35, Issue 2, p595
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1989.28