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- Title
SNP in the Coffea arabica genome associated with coffee quality.
- Authors
Tran, Hue T. M.; Furtado, Agnelo; Vargas, Carlos Alberto Cordero; Smyth, Heather; Slade Lee, L.; Henry, Robert
- Abstract
Association analysis was performed at the whole genome level to identify loci affecting the caffeine and trigonelline content of Coffea arabica beans. DNA extracted from extreme phenotypes was bulked (high and low caffeine, and high and low trigonelline) based on biochemical analysis of the germplasm collection. Sequencing and mapping using the combined reference genomes of C. canephora and C. eugenioides (CC and CE) identified 1351 non-synonymous SNPs that distinguished the low- and high-caffeine bulks. Gene annotation analysis with Blast2GO revealed that these SNPs corresponding to 908 genes with 56 unique KEGG pathways and 49 unique enzymes. Based on KEGG pathway-based analysis, 40 caffeine-associated SNPs were discovered, among which nine SNPs were tightly associated with genes encoding enzymes involved in the conversion of substrates (i.e. SAM, xanthine and IMP) which participate in the caffeine biosynthesic pathways. Likewise, 1060 non-synonymous SNPs were found to distinguish the low- and high-trigonelline bulks. They were associated with 719 genes involved in 61 unique KEGG pathways and 51 unique enzymes. The KEGG pathway-based analysis revealed 24 trigonelline-associated SNPs tightly linked to genes encoding enzymes involved in the conversion of substrates (i.e. SAM, L-tryptophan) which participate in the trigonelline biosynthesis pathways. These SNPs could be useful targets for further functional validation and subsequent application in arabica quality breeding.
- Subjects
COFFEE; TRIGONELLINE; NUCLEIC acid isolation methods; PHENOTYPES; GERMPLASM
- Publication
Tree Genetics & Genomes, 2018, Vol 14, Issue 5, p1
- ISSN
1614-2942
- Publication type
Article
- DOI
10.1007/s11295-018-1282-9