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- Title
Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging.
- Authors
Yuqing Ge; Yu Zhang; Shiying He; Fang Nie; Gaojun Teng; Ning Gu
- Abstract
Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles’ concentration. The minimum detected number of labeled cells was around 104 by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.
- Subjects
NANOPARTICLES; FLUORESCENCE; MAGNETIC resonance imaging; CHITOSAN; CANCER cells; BIOPHYSICAL labeling; MEDICAL imaging systems; TRANSMISSION electron microscopy; FLOW cytometry
- Publication
Nanoscale Research Letters, 2009, Vol 4, Issue 4, p287
- ISSN
1931-7573
- Publication type
Article
- DOI
10.1007/s11671-008-9239-9