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- Title
Highly sensitive and selective detection of selenate in water samples using an enzymatic gold nanodendrite biosensor.
- Authors
Motlagh, Mozhgan Khorasani; Noroozifar, Meissam; Kraatz, Heinz-Bernhard
- Abstract
Gold (Au) and glassy carbon electrodes have been decorated with gold nanodendrites (NDs) using galvanic replacement reactions. Modification of the ND surfaces by lipoic acid N-hydroxysuccinimide ester allows to immobilise selenate reductase to the electrode surface, rendering it selective for the catalytic reduction of selenate ( SeO 4 2 − , Se(VI)) to selenite ( SeO 3 2 − , Se(IV)). Electrode modifications have been characterized by electrochemical impedance spectroscopy and cyclic voltammetry (CV). For selenate detection, CV and differential pulse voltammetry measurements have been carried out in 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid buffer with pH 6.0. Under optimum conditions, the linear range for Au electrodes decorated with AuNDs was 0.3–203 µg/L Se with a limit of detection of 0.01 µg/L Se, which is a 274-fold improvement over using non-nanostructured surfaces for selenate detection. Other anions such as SO 4 2 − , NO 3 - , NO 2 − , PO 4 3 − , AsO 4 3 − , and SeO 3 2 − did not interfere with the detection of selenate. The electrochemical sensor was used for the detection of selenate in three different real samples with recovery between 98.5% and 102.0%.
- Subjects
CARBON electrodes; WATER sampling; WATER use; BIOSENSORS; SUBSTITUTION reactions
- Publication
Canadian Journal of Chemistry, 2023, Vol 101, Issue 7, p440
- ISSN
0008-4042
- Publication type
Article
- DOI
10.1139/cjc-2022-0220