We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Her2/neu Status Determination in Breast Cancer: A Single Institutional Experience Using a Dual-Testing Approach With Immunohistochemistry and Fluorescence In Situ Hybridization.
- Authors
Solomon, James P.; Dell'Aquila, Marie; Fadare, Oluwole; Hasteh, Farnaz
- Abstract
<bold>Objectives: </bold>According to current guidelines, either immunohistochemistry (IHC) or in situ hybridization (ISH) can be used to determine human epidermal growth factor receptor 2 (Her2/neu) status in breast carcinoma. While the guidelines explicitly delineate result interpretation, there is no consensus on the most appropriate testing algorithm.<bold>Methods: </bold>The Her2/neu statuses of 369 consecutive cases of invasive breast cancer (from 351 patients) were assessed in a dual-testing algorithm that uses both IHC and fluorescence ISH (FISH). FISH was performed using dual-color HER2/ chromosome enumeration probe 17 ( CEP17 ) probes, and if equivocal results were obtained, reflex testing using HER2/lissencephaly gene 1 ( LIS1 ) probes was used. Results from both modalities were scored and reported using American Society of Clinical Oncology/College of American Pathologists 2013 criteria.<bold>Results: </bold>Sixty-one (16.5%) of the 369 tumors were found to be Her2/neu positive by at least one modality. The overall concordance between IHC and FISH results was 97.6%. Six of the 369 tumors were reclassified as Her2/neu positive after a negative IHC result. FISH was also able to identify significantly more Her2/neu-positive cases than IHC.<bold>Conclusions: </bold>The commonly used reflex strategy based on IHC results may deny potentially beneficial targeted therapy for a small cohort of patients, which should be considered as testing guidelines are formulated and the cost-benefit analyses of various testing algorithms are assessed.
- Subjects
FLUORESCENCE in situ hybridization; BREAST cancer diagnosis; EPIDERMAL growth factor receptors
- Publication
American Journal of Clinical Pathology, 2017, Vol 147, Issue 4, p432
- ISSN
0002-9173
- Publication type
journal article
- DOI
10.1093/ajcp/aqw224