We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
CD4<sup>+</sup> CCR5<sup>+</sup> and CD4<sup>+</sup> CCR3<sup>+</sup> lymphocyte subset and monocyte apoptosis in patients with acute visceral leishmaniasis.
- Authors
Potestio, Marcella; D'Agostino, Pietro; Romano, Giuseppina Colonna; Milano, Salvatore; Ferlazzo, Viviana; Aquino, Alessandra; Di Bella, Gloria; Caruso, Rosalba; Gambino, Giuseppe; Vitale, Giustina; Mansueto, Serafino; Cillari, Enrico
- Abstract
The potential involvement of apoptosis in the pathogenesis of visceral leishmaniasis (VL) was examined by studying spontaneous andLeishmaniaantigen (LAg)-induced apoptosis using cryopreserved peripheral blood mononuclear cells (PBMC) of Sicilian patients with VL. Results indicate that monocytes and T lymphocytes from acute VL patients show a significantly higher level of apoptosis compared with that observed in healed subjects. The percentage of apoptotic cells was higher in monocytes than in T lymphocytes. T cells involved in programmed cell death (PCD) were mainly of the CD4+ phenotype. In particular, the T helper 1-type (Th1) subset, as evaluated by chemokine receptor-5 (CCR5) expression, is involved in this process. Cell death in Th1-type uses a CD95-mediated mechanism. Furthermore, Th1-type CCR5+ cells are prone to cell suicide in an autocrine or paracrine way, as attested by enhanced expression of CD95L in acute VL patients. The reduction in Th1-type cells by apoptosis was confirmed by the decrease in interferon-γ secretion. In conclusion, apoptosis of monocytes, CD4+ and CD4+ CCR5+ T cells could be involved in the failure of cell mediated immunity that is responsible for severe immune-depression in VL.
- Subjects
MONOCYTES; APOPTOSIS; LEISHMANIASIS; ANTIGENS; LYMPHOCYTES; IMMUNOLOGY
- Publication
Immunology, 2004, Vol 113, Issue 2, p260
- ISSN
0019-2805
- Publication type
Article
- DOI
10.1111/j.1365-2567.2004.01948.x