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- Title
Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets.
- Authors
Yang, H.; Parkhouse, R. M. E.
- Abstract
The swine is a useful model for immunobiological studies as it has a highly heterogeneous lymphocyte pool, containing several subsets not easily accessible in humans and rodents. In particular, the CD8-positive (CD8+) cells contain a variety of lymphocyte subsets, such as αβ-T cells, γδ-T cells, CD4 CD8 double-positive (DP) cells and natural killer (NK) cells. In order to define these subsets further, we have selected four monoclonal antibodies (mAb) with differential reactivity on CD8+ cells. Thus, mAb CD8.1 (PPT20) bound to CD8hi and CD8lo subpopulations in a similar way to the conventional anti-CD8. The mAb CD8.2 (PPT21), though binding to all of the CD8+ cells, reacted preferably with CD8hi. Two other mAb, CD8.3 (PPT22) and CD8.4 (PPT23), were specific for CD8hi αβ-T-cell subpopulation. These results, complemented by immunoprecipitation, co-modulation and enzyme-linked immunosorbent assay experiments, suggest that CD8.1 and CD8.2 react putatively with the CD8α-chain and CD8.3 and CD8.4 with the CD8β-chain. Tissue distribution studies revealed that CD8+ thymocytes and peripheral CD8hi αβ-T cells expressed both putative CD8α- and β-chains while peripheral CD4+CD8+ αβ-T cells, CD8lo γδ-T cells and NK cells expressed only putative CD8α-chain. Functional studies indicated that the CD8hi αβ-T and CD8lo γδ-T cells were effector cells in the CD3-redirected cytotoxicity.
- Subjects
CD antigens; EPITOPES; LYMPHOCYTES; T cells; ENZYME-linked immunosorbent assay; MONOCLONAL antibodies
- Publication
Immunology, 1997, Vol 92, Issue 1, p45
- ISSN
0019-2805
- Publication type
Article
- DOI
10.1046/j.1365-2567.1997.00308.x