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- Title
Microbial colonization influences composition and T-cell receptor V/β repertoire of intraepithelial lymphocytes in rat intestine.
- Authors
Helgeland, L.; Vaage, J. T.; Rolstad, B.; Midtvedt, T.; Brandtzaeg, P.
- Abstract
Studies in mice have shown that the composition of intestinal intraepithelial lymphocytes (IEL) may be markedly altered by gut microbial colonization. Such modulation was studied in a rat model by the use of germ-free and conventionalized animals from which IEL from the small intestine were isolated and analysed by flow cytometry. Conventionalization caused expansion as well as phenotypic alterations of T-cell receptor (TCR) α/β+ IEL in that the proportions of CD4+ and CD8αβ+ TCRβ/β+ cells were increased, while the double negative (CD4- CD8-) fraction was reduced. Microbial colonization also influenced the TCR Vβ repertoire of CD8+ IEL in that the proportions of Vβ8.2+ and Vβ10+ cells were increased, whereas Vβ8.5+ and Vβ16+ cells were relatively decreased. Moreover, conventionalization influenced the levels of TCR cell surface expression in the same Vβ subsets. Three-colour flow-cytometric analysis demonstrated that skewing of the Vβ repertoire was most pronounced in the CD8αα+ subset, although the numerical increase of IEL mainly included the CD8αβ+ subset. In contrast to IEL, the TCR Vβ repertoire in mesentexic lymph nodes was unchanged after intestinal colonization. These results confirm that TCRα/β+ IEL subpopulations respond dynamically to the microbial gut flora and suggest that their Vβ repertoire can be shaped by luminal microbial antigens.
- Subjects
BINDING sites; IMMUNOGLOBULINS; IMMUNITY; LEUCOCYTES; LYMPHOCYTES; CELL receptors; LYMPH nodes; ANTIGENS; CELL membranes
- Publication
Immunology, 1996, Vol 89, Issue 4, p494
- ISSN
0019-2805
- Publication type
Article
- DOI
10.1046/j.1365-2567.1996.d01-783.x