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- Title
A Critical Interaction between NR2B and MAGUK in L-DOPA Induced Dyskinesia.
- Authors
Gardoni, Fabrizio; Picconi, Barbara; Ghiglieri, Veronica; Polli, Federica; Bagetta, Vincenza; Bernardi, Giorgio; Cattabeni, Flaminio; Di Luca, Monica; Calabresi, Paolo
- Abstract
Abnormal function of NMDA receptor has been suggested to be correlated with the pathogenesis of Parkinson's disease (PD) as well as with the development of L-3,4-dihydroxyphenylalanine (L-DOPA)-induced dyskinesia. Here we show that NMDA receptor NR2 subunits display specific alterations of their subcellular distribution in striata from unilateral 6-hydroxydopamine-lesioned, L-DOPA-treated dyskinetic, and L-DOPA-treated nondyskinetic rats. Dyskinetic animals have significantly higher levels of NR2A subunit in the postsynaptic compartment than all other experimental groups, whereas NR2B subunit shows a significant reduction in both dopamine-denervated and dyskinetic rats. These events are paralleled by profound modifications of NMDA receptor NR2B subunit association with interacting elements, i.e., members of the membrane-associated guanylate kinase (MAGUK) protein family postsynaptic density-95, synapse-associated protein-97 and synapse-associated protein-102. Treatment of nondyskinetic animals with a synthetic peptide (TAT2B) able to affect NR2B binding to MAGUK proteins as well as synaptic localization of this subunit in nondyskinetic rats was sufficient to induce a shift of treated rats toward a dyskinetic motor behavior. These data indicate abnormal NR2B redistribution between synaptic and extrasynaptic membranes as an important molecular disturbance of the glutamatergic synapse involved in L-DOPA-induced dyskinesia.
- Subjects
METHYL aspartate; PARKINSON'S disease; MOVEMENT disorders; RATS; DOPAMINE
- Publication
Journal of Neuroscience, 2006, Vol 26, Issue 11, p2914
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.5326-05.2006