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- Title
Human papillomavirus genotyping and integration in ovarian cancer Saudi patients.
- Authors
Al-Shabanah, Othman A.; Hafez, Mohamed M.; Hassan, Zeinab K.; Sayed-Ahmed, Mohamed M.; Abozeed, Waleed N.; Al-Rejaie, Salem S.; Alsheikh, Abdulmalik A.
- Abstract
Background Human papillomavirus (HPV) is associated with different malignancies butits role in the pathogenesis of ovarian cancer is controversial. This study investigatedthe prevalence, genotyping and physical state of HPV in ovarian cancer Saudi patients. Methods Hundred formalin fixed paraffin embedded (FFPE) ovarian carcinoma tissues and their normal adjacent tissues(NAT) were included in the study.HPV was detected by nested polymerase chain reaction (PCR) using degenerated HPVL1 consensus primer pairs MY09/MY11 and GP5+/GP6 + to amplify a broad spectrum of HPV genotypes in a single reaction. The HPV positive samples were furthergenotyped using DNA sequencing. The physical state of the virus was identified using Amplification of Papillomavirus Oncogene Transcripts (APOT) assay in the samples positive for HPV16 and/or HPV18. Results High percentage of HPV (42%) was observed in ovarian carcinoma compared to 8% in the NAT. The high-risk HPV types 16, 18 and 45 were highly associated with the advanced stages of tumor, while low-risk types 6 and 11 were present inNAT. In malignant tissues, HPV-16was the most predominant genotype followed by HPV-18and -45.The percentage of viral integration into the host genome was significantly high (61.1%) compared to 38.9% episomal in HPV positive tumors tissues. In HPV18 genotypethe percentage of viral integration was 54.5% compared to 45.5% episomal. Conclusion The high risk HPV genotypes in ovarian cancer may indicate its role in ovarian carcinogenesis. The HPV vaccination is highly recommended to reduce this type of cancer.
- Subjects
PAPILLOMAVIRUSES; OVARIAN cancer; POLYMERASE chain reaction; NUCLEOTIDE sequence; CARCINOGENESIS
- Publication
Virology Journal, 2013, Vol 10, Issue 1, p1
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/1743-422X-10-343