We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Evaluación de la citotoxicidad y bioseguridad de un extracto de polifenoles de huesos de aceitunas.
- Authors
Galindo, C. Veciana; Cortés Castell, E.; Torro Montell, L.; Sirvent Segura, E.; Rizo Baeza, M. M.; Gil Guillén, V.
- Abstract
The olive tree is a source of bioactive compounds, both its fruit and its by-products. Some of its compounds have shown health benefits, being objective of this work the evaluation of biosafety in-vitro and in vivo of extracts of olive stones rich in polyphenols. Material and methods: He has been evaluated for cytotoxicity by addition of lyophilized extract dissolved in PBS(0-400 mg/l) to a culture of the cell line THP1-XBlue- CD14 and evaluation of cell viability by the reaction of reduction of resazurin by living cells. Biosecurity has been evaluated in zebrafish, incubating eggs fertilized in 0 to 100 mg/l extract for 24 to 72 hours and measuring parameters: a) lethal (dead embryos, coagulated eggs), b) sublethal (spontaneous movements, pigmentation, edemas) and c) teratogenic (malformations, retardation development). Results: Cytotoxicity (toxic effect when less than 75% viability) extract bones of olive in the cell line THP1- XBlue-CD14, is in concentrations higher than 50 mg/l, calculating a LD50 (dose lethality 50) more than 800 mg/l. The biosafety of zebrafish embryos exposed to concentrations of extract from 0-100 mg/l showed total viability at 24, 48 and 72 hours post fertilization (hpf), not observed mortality or appreciated embryos with sublethal effects, teratogenic, or advancement or delay in hatching. It can be concluded that the bones of olive extract is highly biosecured until at least 100 mg/l concentrations.
- Subjects
CELL-mediated cytotoxicity; ZEBRA danio; BIOACTIVE compounds; PLANT products; BIOSAFETY; BIOSECURITY
- Publication
Nutrición Hospitalaria, 2014, Vol 29, Issue 6, p1388
- ISSN
0212-1611
- Publication type
Article
- DOI
10.3305/nh.2014.29.6.7141