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- Title
ADP-Ribosyl Proteins Formed by Pertussis Toxin are Specifically Cleaved by Mercury Ions.
- Authors
MEYER, Thomas; KOCH, Robert; FANICK, Werner; HILZ, Helmuth
- Abstract
Various types of ADP-ribosyl protein conjugates were synthesized and their chemical stability was compared with that of cysteinelinked ADP-ribosyl groups as formed by incubation of transducin or Gi/Go proteins with NAD and pertussis toxin. Treatment with 0.1 mM HgCl2 specifically cleaved the cysteine-linked conjugates. This may provide a tool for the quantitation of modified Gi/Go proteins as well as of other acceptors modified by ADP-ribose at cysteine residues in the presence of other ADP-ribosyl proteins.
- Publication
Biological Chemistry, 1988, Vol 369, Issue 2, p579
- ISSN
1431-6730
- Publication type
Article