We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Nitrogen Fertilization Changes Abundance and Community Composition of Ammonia-Oxidizing Bacteria.
- Authors
Weishou Shen; Xiangui Lin; Nan Gao; Weiming Shi; Ju Min; Xinhua He
- Abstract
The effects of N fertilization rates on the abundance and community composition of NH3-oxidizing bacteria (AOB) are poorly understood, particularly in greenhouse-based high-N-input vegetable soils. The abundance and community composition of AOB from these vegetable soils under five N (urea) rates in eastern China were evaluated by polymerase chain reaction (PCR) and denaturing gradient gel dectrophoresis (DGGE). These rates were: the conventional high N rate (N4) as 270 or 300 kg N ha-1 season-1 for cucumber (Cucumis sativus L.) or tomato (Solanum lycopersicum L.), respectively, and 80% (N3), 60% (N2), 40% (N1), and 0% (N0) of the N4 rate. ARer 2 yr of a cucumber-tomato rotation, N fertilization }lad significant effects on the abundance and community com- position of AOB. The abundance ofAOB amo,4 gene was significantly higher under the N2 (2.08 x 1010 kg-1 dry weight soil) than under the NO or N4 (8.74 or 8.75 x 109 kg-1 soil). The DGGE patterns were more diverse under N1 and N2 than under N3 and N4, with the greatest DGGE band numbers (15) under N2. Phylogenetic analyses of amo A gene fragments showed that AOB sequences from different N fertilization rates were affiliated with Nitrosospira species and grouped into Clusters 2, 3, 8, and 12. Our results indicate that a reduction to 40% of the conventional high N rate (N4) is practical to reduce N inputs while maintaining the abundance and community composition of AOB in such greenhouse-based vegetable soils.
- Subjects
AMMONIA-oxidizing bacteria; NITROGEN fertilizers; GEL electrophoresis; SOIL testing; POLYMERASE chain reaction
- Publication
Soil Science Society of America Journal, 2011, Vol 75, Issue 6, p2198
- ISSN
0361-5995
- Publication type
Article
- DOI
10.2136/sssaj2010.0459