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- Title
Cellular localization of Visudyne<sup>®</sup> as a function of time after local injection in an in vivo model of squamous cell carcinoma: an investigation into tumor cell death.
- Authors
Barnes, Laura D.; Giuliano, Elizabeth A.; Ota, Juri
- Abstract
Objective To evaluate the effects of time on cellular localization of Visudyne® after local injection. Animals Twenty athymic nude mice. Procedures A squamous cell carcinoma (SCC) cell line (A-431) was injected into right and left dorsolumbar subcutaneous tissue of each mouse, representing treatment (T) and control (C) tumors. In experiment 1 (Exp 1; n = 10) and 2 (Exp 2; n = 10), the T tumors received a local injection of Visudyne® (0.1 mg/cm3), and C tumors received an equal dose of 5% dextrose in water (D5W). Mice were randomly subdivided into two groups (A and B; n = 5 per group). Mice in Exp 1A and B were sacrificed 1 and 30 min after local injection, respectively. Experiment 1A and B tumors were evaluated by fluorescence microscopy to determine drug localization. Experiment 2A and B tumors were exposed to LED illumination 1 and 30 min after injection, respectively, and evaluated by transmission electron microscopy (TEM) to determine ultrastructural tumor cell damage. Results Fluorescence was detected within the cytoplasm of T tumors in both Exp 1A and B. Significance was detected in fluorescence intensity between T1 min vs. T30 min ( P = 0.03) and between T1 min and C1 min tumors ( P = 0.01), respectively. Tumors in Exp 2A and B demonstrated evidence of apoptotic cell death. Conclusions Fluorescence microscopy demonstrated higher Visudyne® concentration within SCC cytoplasm of 1 min compared with 30-min tumors. Transmission electron microscopy results revealed that tumors treated by photodynamic therapy (PDT) within 30 min of local injection undergo cellular apoptosis.
- Subjects
SQUAMOUS cell carcinoma; MICE; DEXTROSE; FLUORESCENCE microscopy; TRANSMISSION electron microscopy; CELL death
- Publication
Veterinary Ophthalmology, 2010, Vol 13, Issue 3, p158
- ISSN
1463-5216
- Publication type
Article
- DOI
10.1111/j.1463-5224.2010.00775.x