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- Title
The ArabidopsisERECTAgene is expressed in the shoot apical meristem and organ primordia.
- Authors
Yokoyama, Ryusuke; Takahashi, Taku; Kato, Atsushi; Torii, Keiko U.; Komeda, Yoshibumi
- Abstract
Summary:InArabidopsis thaliana(L.) Heynh, the mutation inERECTAis known to confer a compact inflorescence by a reduction in the lengths of internodes and pedicels. We analyzed the expression pattern of this gene during plant development.In situhybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with theERECTApromoter fused to the β-glucuronidase (GUS) gene, showed thatERECTAwas predominantly expressed in the shoot apical meristems and organ primordia.ERECTAexpression in the shoot apical meristem was weak early in plant development but increased with the transition from the vegetative to the reproductive growth phase.ERECTAwas also strongly expressed in organ primordia and immature organs but weakly in mature organs. Thus,ERECTAwas expressed in a cell-specific and developmentally regulated manner. In order to identify the regulatory mechanism responsible for the expression pattern ofERECTA, thecis-acting regions in theERECTApromoter were defined by study of the expression of the chimeric genes that consist of the 5′- or internal deleted promoter and a GUS reporter gene in transgenic plants. The results showed that the essentialcis-regulatory elements governing the spatially and temporally specific expression ofERECTAare located between positions –462 and – 228 bp and between positions –228 and –153 bp with respect to the transcriptional initiation site..
- Subjects
GENE expression in plants; ARABIDOPSIS thaliana; GENETICS
- Publication
Plant Journal, 1998, Vol 15, Issue 3
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1046/j.1365-313X.1998.00203.x