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- Title
KESTOSE, A PREBIOTIC FRUCTOOLIGOSACCHARIDE, ENHANCES INTERCELLULAR TIGHT JUNCTION RECOVERY VIA A RHO-ASSOCIATED KINASE-DEPENDENT MECHANISM IN INTESTINAL CACO-2 CELLS.
- Authors
Toshimitsu Shirai; Yoshimitsu Suzuki; Lakohei Kamikado; Yasuhiro Koga; Ryo Aoki
- Abstract
Kestose, a prebiotic fructooligosaccharide, has been shown to exert a therapeutic effect on the cBnical manifestation of atopic dermatitis (AD) in infants through a unknown mechanisms. Since restoration of the impaired intestinal barrier may be useful for the treatment of AD, we investigated the effect of kestose on the intestinal barrier using the Caco-2 cell monolayer in a Ca2+ switch assay. Our results show that apical kestose treatment (1% w/v) led a significant acceleration of the restoration of barrier function after Ca2+ replenishment. Kestose also enhanced the reassembly of tight junction (TJ) proteins. An acceleration in the deposphorylation of myosin light chain (MLC) was also found by kestose treatment. Moreover, an inhibitor of Rho-associated kinase (ROCK), a key enzyme involved in the phosphorylation of MLC, abrogated the kestose-mediated promotion of TJ restoration. These results indicate that kestose accelerates the recovery of epithelial TJ assembly through the induction of MLC dephosphorylation via a ROCK-dependent mechanism.
- Subjects
FRUCTOOLIGOSACCHARIDES; PREBIOTICS; FUNCTIONAL foods; FUNCTIONAL beverages; ATOPIC dermatitis; MONOMOLECULAR films
- Publication
International Journal of Probiotics & Prebiotics, 2013, Vol 8, Issue 2/3, p53
- ISSN
1555-1431
- Publication type
Article