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- Title
Development of candidate biomarkers for pancreatic ductal adenocarcinoma using multiple reaction monitoring.
- Authors
Yu, Jiyoung; Kim, Kyunggon; Kang, MeeJoo; Kim, Hyunsoo; Kim, Sun; Jang, Jin-Young; Kim, Youngsoo
- Abstract
Pancreatic ductal adenocarcinoma (PDAC) is the fourth most frequent cause of cancer mortality in the United States. Because CA 19-9 increases not only in PDAC, but also in benign conditions, there is urgent need for an additional PDAC biomarker. Isotope tags for relative and absolute quantification (iTRAQ) were performed using 6 pairs of PDAC and normal tissues from the same patients, to obtain preliminary PDAC-specific proteins; and verification was performed by multiple reactions monitoring (MRM), using 30 PDAC and 20 normal serum, targeting high-abundant serum proteins without any pre-preparation. As a result, 17 candidate proteins from tissue iTRAQ were verified as potential markers (AUC values > 0.7). Multivariate analysis (MA) demonstrated that a 6-marker panel, consisting of alpha-1 antitrypsin, haptoglobin beta chain, hemopexin, transferrin, zinc alpha-2 glycoprotein, and apolipoprotein A4 from the MRM result, had comparable discriminatory power versus CA 19-9. Our study demonstrated that a combination of iTRAQ on PDAC tissue and verification MRM-MA on individual serum was an efficient method for the development of PDAC multimarkers.
- Subjects
PANCREATIC cancer; BIOMARKERS; ADENOCARCINOMA; PROTEOMICS; GLYCOPROTEINS; TRANSFERRIN
- Publication
Biotechnology & Bioprocess Engineering, 2013, Vol 18, Issue 5, p1038
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-013-0421-2