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- Title
A diet containing fish oil and pectin ameliorates radiation-enhanced colon carcinogenesis by suppression of PPAR<sub>δ</sub> and PGE synthase-2 (PGES<sub>2</sub>) and elevation of PGE<sub>3</sub>.
- Authors
Vanamala, J.; Glagolenko, A.; Yang, P.; Carroll, R. J.; Murphy, M. E.; Newman, R. A.; Chapkin, R. S.; Turner, N. D.; Lupton, J. R.
- Abstract
Radiation-enhanced carcinogenesis is a major impediment to long-duration space flight that dietary countermeasures may potentially ameliorate. We have shown that dietary fish oil/pectin (FP) may protect against colon cancer by enhancing spontaneous colonocyte apoptosis. To investigate the mechanism of action, we provided rats (n = 40) with diets containing the combination of FP or corn oil/cellulose (CC). Rats were exposed to 0 or 1 Gy, 1 GeV/nucleon Fe-ion, 3 wk after starting the diets. All rats were injected with AOM (15 mg/kg), 10 and 17 d after irradiation. We measured the levels of colonocyte apoptosis (TUNEL), PGES2 and PPARδ (Westerns), and PGE2 and PGE3 (LC/MS/MS) 31 wk after the last AOM injection. The FP induced higher (P<0.01) apoptotic indices which are associated with suppression of the anti-apoptotic mediators PGES2 (P=0.045), PGE2 (P=0.0001), and PPARδ (P=0.0085) compared to the CC diet in AOM only and AOM + radiation rats. Moreover, FP induced greater (P<0.01) pro-apoptotic PGE3 in irradiated rats. These results indicate that FP counteracted the radiation elevated (P=0.045) anti-apoptotic mediators (PPARδ and PGES2) by upregulating (P=0.033) pro-apoptotic PGE3 levels. Thus, the FP diet may protect against radiation-enhanced suppression of apoptosis during colon carcinogenesis via elevation of PGE2 levels.
- Subjects
FISH oils; PECTINS; RADIATION carcinogenesis; NUCLEAR receptors (Biochemistry); PROSTAGLANDINS E
- Publication
FASEB Journal, 2007, Vol 21, Issue 5, pA166
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.5.a166