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- Title
Development of cost-effective tetra-ARMS PCR for detection of FecB genotype in sheep.
- Authors
Feng Guan; Guoqing Shi; Pengcheng Wan; Rong Dai; Hong Tang; Haixia Wang; Yuanyuan Luo
- Abstract
The FecB gene has been shown to be crucial in reproduction in many sheep breeds. It is a single nucleotide polymorphism (SNP) located in the bone morphogenetic protein receptor IB (BMPR-IB) gene. The current methods for genotyping the FecB mutation are either slow and laborious or expensive. In this report, a single-step amplification approach suitable for FecB genotyping method is described. Multiplex PCR was performed with four primers on the basis of tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS PCR), then three FecB genotypes can be detected after electrophoresis. Genotyping results of the proposed multiplex PCR occurred to be in complete accordance with forced PCR-RFLP of all samples. It is a rapid and simple method for detection of FecB in a larger number of samples, and is suitable for other SNPs detection with specific primers even in most "low-tech" laboratories.
- Subjects
COST effectiveness; POLYMERASE chain reaction; SHEEP genetics; SHEEP breeds; MAMMAL reproduction; SHEEP; SINGLE nucleotide polymorphisms; BONE morphogenetic protein receptors
- Publication
Animal Science Papers & Reports, 2014, Vol 32, Issue 3, p229
- ISSN
0860-4037
- Publication type
Article