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- Title
The yeast aquaglyceroporin Fps1p is a bidirectional arsenite channel
- Authors
Maciaszczyk-Dziubinska, Ewa; Migdal, Iwona; Migocka, Magdalena; Bocer, Tomasz; Wysocki, Robert
- Abstract
Abstract: The stress-activated kinase Hog1p mediates arsenic tolerance by decreasing arsenite influx through the aquaglyceroporin Fps1p in Saccharomyces cerevisiae. Unexpectedly, we found that overexpression of FPS1 increased arsenite tolerance suggesting a physiological role of Fps1p in arsenic detoxification. Consistently, during arsenite treatment transcription of FPS1 gene was strongly upregulated, while Fps1p was not degraded and remained localized to the plasma membrane. Moreover, deletion of FPS1 gene resulted in arsenate sensitivity. Finally, transport experiments revealed that Fps1p in concert with the arsenite transporter Acr3p mediates arsenite efflux.
- Subjects
AQUAPORINS; YEAST fungi biotechnology; PHYSIOLOGICAL effects of arsenic; ION channels; SACCHAROMYCES cerevisiae; CELL membranes; PROTEIN kinases; GENE expression
- Publication
FEBS Letters, 2010, Vol 584, Issue 4, p726
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.12.027