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- Title
Characterization of human thioredoxin-like-1: Potential involvement in the cellular response against glucose deprivation
- Authors
Jiménez, Alberto; Pelto-Huikko, Markku; Gustafsson, Jan-Åke; Miranda-Vizuete, Antonio
- Abstract
Abstract: The thioredoxin system, composed of thioredoxin (Trx) and thioredoxin reductase (TrxR), emerges as one of the most important thiol-based systems involved in the maintenance of the cellular redox balance. Thioredoxin-like-1 (TXL-1) is a highly conserved protein comprising an N-terminal Trx domain and a C-terminal domain of unknown function. Here we show that TXL-1 is a substrate for the cytosolic selenoprotein TrxR-1. In situ hybridization experiments demonstrates high expression of Txl-1 mRNA in various areas of central nervous system and also in some reproductive organs. Glucose deprivation, but not hydrogen peroxide treatment, reduced the levels of endogenous TXL-1 protein in HEK-293 cell line. Conversely, overexpression of TXL-1 protects against glucose deprivation-induced cytotoxicity. Taken together, the finding that Txl-1 mRNA is highly expressed in tissues which use glucose as a primary energy source and the modulation of TXL-1 levels upon glucose deprivation indicate that TXL-1 might be involved in the cellular response to sugar starvation stress.
- Subjects
THIOREDOXIN; GLUCOSE; PROTEINS; MESSENGER RNA
- Publication
FEBS Letters, 2006, Vol 580, Issue 3, p960
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.01.025