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- Title
Alisol A 24-acetate stimulates lipolysis in 3 T3-L1 adipocytes.
- Authors
Lou, Hai-xia; Fu, Wen-cheng; Chen, Jia-xiang; Li, Tian-tian; Jiang, Ying-ying; Liu, Chun-hui; Zhang, Wen
- Abstract
Background: Alisol A 24-acetate (AA-24-a), one of the main active triterpenes isolated from the well-known medicinal plant Alisma orientale (Sam.) Juz., exhibits multiple biological activities including hypolipidemic activity. However, its effect on lipid metabolism in adipocytes remains unclear. The present study aimed to clarify the effect of AA-24-a on adipocyte lipolysis and to determine its potential mechanism of action using 3 T3-L1 cells. Methods: We assayed the release of glycerol into culture medium of 3 T3-L1 cells under treatment with AA-24-a. Protein and mRNA expression and phosphorylation levels of the main lipases and kinases involved in lipolysis regulation were determined by quantitative polymerase chain reaction and western blotting. Specific inhibitors of protein kinase A (PKA; H89) and extracellular signal-regulated kinase (ERK; PD98059), which are key enzymes in relevant signaling pathways, were used to examine their roles in AA-24-a-stimulated lipolysis. Results: AA-24-a significantly stimulated neutral lipolysis in fully differentiated adipocytes. To determine the underlying mechanism, we assessed the changes in mRNA and protein levels of key lipolysis-related genes in the presence or absence of H89 and PD98059. Both inhibitors reduced AA-24-a-induced lipolysis. Moreover, pretreatment with H89 attenuated AA-24-a-induced phosphorylation of hormone-sensitive lipase at Ser660, while pretreatment with PD98059 attenuated AA-24-a-induced downregulation of peroxisome proliferator-activated receptor-γ and perilipin A. Conclusions: Our results indicate that AA-24-a promoted neutral lipolysis in 3 T3-L1 adipocytes by activating PKA-mediated phosphorylation of hormone-sensitive lipase and ERK- mediated downregulation of expression of perilipin A.
- Subjects
LIPID metabolism; OBESITY; LIPASES; PROTEIN kinases; TERPENES; FIBROBLASTS; ANIMAL experimentation; WESTERN immunoblotting; ONE-way analysis of variance; MICRORNA; PEROXISOME proliferator-activated receptors; CELLULAR signal transduction; GENE expression; FAT cells; TRANSFERASES; DESCRIPTIVE statistics; PLANT extracts; CELL lines; MOLECULAR structure; POLYMERASE chain reaction; DATA analysis software; PHOSPHORYLATION; CARRIER proteins
- Publication
BMC Complementary Medicine & Therapies, 2021, Vol 21, Issue 1, p1
- ISSN
2662-7671
- Publication type
Article
- DOI
10.1186/s12906-021-03296-0