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- Title
DdCBE-mediated mitochondrial base editing in human 3PN embryos.
- Authors
Chen, Xiaoxu; Liang, Dong; Guo, Jiayin; Zhang, Junqiang; Sun, Haifeng; Zhang, Xiaolan; Jin, Jiachuan; Dai, Yichen; Bao, Qinmin; Qian, Xuezhen; Tan, Lei; Hu, Ping; Ling, Xiufeng; Shen, Bin; Xu, Zhengfeng
- Abstract
In recent years, advances in genome editing technologies have been achieved for precise editing of the nuclear genes, whereas due to the lack of repair mechanism for mtDNA, it is technically challenging to precisely edit mtDNA[4]. Since DdCBE-mediated base conversion relies on the replication of mtDNA after deamination, inactive mtDNA replication in early embryonic stages would impair the editing outcome. 4 Bacman SR. MitoTALEN reduces mutant mtDNA load and restores tRNA(Ala) levels in a mouse model of heteroplasmic mtDNA mutation. To profile the off-target activity of DdCBEs on the entire mitochondrial genome, we first performed whole mtDNA sequencing in HEK293FT cells.
- Subjects
HUMAN embryos; MITOCHONDRIAL DNA; GENOME editing; MITOCHONDRIA
- Publication
Cell Discovery, 2022, Vol 8, Issue 1, p1
- ISSN
2056-5968
- Publication type
Letter
- DOI
10.1038/s41421-021-00358-y