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- Title
Anti-Inflammatory Effects of Diospyrin on Lipopolysaccharide-Induced Inflammation Using RAW 264.7 Mouse Macrophages.
- Authors
Shahidullah, Adnan; Lee, Ji-Young; Kim, Young-Jin; Halimi, Syed Muhammad Ashhad; Rauf, Abdur; Kim, Hyun-Ju; Kim, Bong-Youn; Park, Wansu
- Abstract
Diospyrin is a bisnaphthoquinonoid medicinal compound derived from Diospyros lotus, with known anti-cancer, anti-tubercular, and anti-leishmanial activities against Leishmania donovani. However, the effects of diospyrin on lipopolysaccharide (LPS)-induced macrophage activation and inflammation are not fully reported. In this study, the anti-inflammatory effects of diospyrin on LPS-induced macrophages were examined. Diospyrin showed no toxicity in RAW 264.7 at concentrations of up to 10 μM. Diospyrin moderated the production of nitric oxide (NO), monocyte chemotactic protein-1, macrophage inflammatory protein-1β, interleukin (IL)-6, IL-10, granulocyte colony-stimulating factor, granulocyte macrophage colony-stimulating factor, vascular endothelial growth factor, leukemia inhibitory factor, and RANTES/CCL5, as well as calcium release in LPS-induced RAW 264.7, at concentrations of up to 10 μM significantly (p < 0.05). Diospyrin also significantly inhibited the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and mRNA expression of C/EBP homologous protein (CHOP), as well as tumor necrosis factor receptor superfamily member 6 (Fas), in LPS-induced RAW 264.7 cells at concentrations of up to 10 μM (p < 0.05). Diospyrin exhibits anti-inflammatory properties mediated via inhibition of NO, and cytokines in LPS-induced mouse macrophages via the ER-stressed calcium-p38 MAPK/CHOP/Fas pathway.
- Subjects
TUMOR necrosis factor receptors; LEUKEMIA inhibitory factor; MACROPHAGE colony-stimulating factor; VASCULAR endothelial growth factors; MITOGEN-activated protein kinases; MACROPHAGE inflammatory proteins; ANTIPARASITIC agents
- Publication
Biomedicines, 2020, Vol 8, Issue 1, p11
- ISSN
2227-9059
- Publication type
Article
- DOI
10.3390/biomedicines8010011