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- Title
Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence.
- Authors
Guochao Zheng; Alsarakibi, Muhamd; Yuanjia Liu; Wei Hu; Qin Luo; Liping Tan; Guoqing Li
- Abstract
This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), ß-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.
- Subjects
GUANGDONG Sheng (China); GIARDIA; PROTOZOA genetics; LOCUS (Genetics); NUCLEOTIDE sequence; POLYMERASE chain reaction; GLUTAMATE dehydrogenase
- Publication
Korean Journal of Parasitology, 2014, Vol 52, Issue 3, p299
- ISSN
0023-4001
- Publication type
Article
- DOI
10.3347/kjp.2014.52.3.299