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- Title
Calpains are Involved in Entamoeba histolytica-lnduced Death of HT-29 Colonic Epithelial Cells.
- Authors
Yun Soo Jang; Kyoung-Ju Song; Ju Young Kim; Young Ah Lee; Kyeong Ah Kim; Sang Kyou Lee; Myeong Heon Shin
- Abstract
Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and μ-calpain by siRNA significantly reduced E. histo/ytica-induced HT-29 cell death. These results suggest that m- and μ-calpain may be involved in colon epithelial cell death induced by E. histolytica.
- Subjects
ENTAMOEBA histolytica; CELL death; PROTOZOA; COLITIS; EPITHELIAL cells; AMOEBA; CALPAIN
- Publication
Korean Journal of Parasitology, 2011, Vol 49, Issue 2, p177
- ISSN
0023-4001
- Publication type
Article
- DOI
10.3347/kjp.2011.49.2.177