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- Title
In vitro anti-HBs antibody synthesis from anti-hepatitis B vaccine recipients.
- Authors
Barnaba, V.; Levrero, M.; Ruberti, Giolvina; van Dyke, Arlene; Perrone, A.; Musca, A.; Balsano, F.
- Abstract
Peripheral blood mononuclear cells (PBMC) from 'responders' recently boosted with hepatitis B vaccine, were studied for synthesis in vitro of antibody to hepatitis B surface antigen (anti-HBs Ab) when stimulated with pokeweed mitogen (PWM) or HBsAg. HBsAg alone can induce an antigen-specific anti-HBs Ab response in vitro; this antibody synthesis is T cell-dependent. In some responders both allogeneic T4+ cells (in absence of PWM or HBsAg) and mixed leucocyte culture supernatants (MLC/SN) (without T cells and antigen) can help responder B cells to produce anti-HBs Ab. Thus, in some immunized subjects, B lymphocytes involved in anti-HBs Ab synthesis are in an advanced phase of differentiation and require only non-antigen specific T cell signals (B cell growth factor or B cell differentiation factor or interleukin 2, etc) to differentiate into antibody- secreting cells. Moreover, the concentration of the antigen necessary to suppress anti-HBs Ab production induced by HBsAg was five times lower than that necessary to suppress antibody production induced by PWM. T cell help for antigen induced anti-HBs Ab could be different from T cell help for the PWM-induced anti-HBs Ab response. Moreover, the finding that the low HBsAg doses inhibiting specific response did not affect the PWM- driven anti-HBs response suggests that antigen-specific T suppressor cells could play a role in this context.
- Subjects
LYMPHOCYTES; LIVER diseases; T cells; B cells; HEPATITIS B vaccines; PREVENTIVE medicine
- Publication
Clinical & Experimental Immunology, 1987, Vol 70, Issue 2, p283
- ISSN
0009-9104
- Publication type
Article