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- Title
Donor-specific DNA - a noninvasive biomarker of renal allograft failure.
- Authors
Moise, A.; Constantinescu, I.; Sinescu, R. D.; Gîngu, C.; Sinescu, I.
- Abstract
Introduction: Renal transplantation is the most effective treatment for most patients with chronic renal failure, significantly improving quality of life. However, about 15-20% of patients undergo at least one episode of acute rejection, that can be fatal for allograft survival. Currently, accurate diagnosis is obtained by percutaneous renal biopsy, an intervention with the potential for secondary complications. Objectives: In our study we aimed to detect the presence of post-transplant donor-specific DNA in the serum of the recipient and establish a possible correlation between this and episodes of rejection. Material and methods: We selected a total of 40 kidney transplanted patients in 2011 in Fundeni Clinical Institute (16 with cadaveric donor and 24 with living donor). Detection of donor-specific DNA was achieved by molecular biology methods (SSP-sequence specific primers), following the presence / absence of donor specific HLA-DRB1. Results: Post-transplant, on day three, donor-specific DNA was detected in nine (56%) of recipients with cadaveric donor and only seven (29%) of recipients with living donor. One month postransplant, only three patients presented donor-derived DNA in serum. Tests carried out during rejection episodes showed the presence of donor DNA in seven of ten cases. Conclusion: Because it is a noninvasive biomarker, accessible for laboratories of immunogenetics involved in posttransplant monitoring, donor-specific DNA detection in recipient serum can be a biomarker for early diagnosis of renal allograft damage.
- Subjects
BIOMARKERS; DNA; UROLOGY; HOMOGRAFTS; BIOCHEMISTRY
- Publication
Romanian Journal of Urology, 2012, Vol 11, Issue 3, p13
- ISSN
1223-0650
- Publication type
Article