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- Title
Longitudinal Study of Cellular and Systemic Cytokine Signatures to Define the Dynamics of a Balanced Immune Environment During Disease Manifestation in Zika Virus-Infected Patients.
- Authors
Lum, Fok-Moon; Lye, David C B; Tan, Jeslin J L; Lee, Bernett; Chia, Po-Ying; Chua, Tze-Kwang; Amrun, Siti N; Kam, Yiu-Wing; Yee, Wearn-Xin; Ling, Wei-Ping; Lim, Vanessa W X; Pang, Vincent J X; Lee, Linda K; Mok, Esther W H; Chong, Chia-Yin; Leo, Yee-Sin; Ng, Lisa F P
- Abstract
<bold>Background: </bold>Since its unexpected reemergence, Zika virus (ZIKV) has caused numerous outbreaks globally. This study characterized the host immune responses during ZIKV infection.<bold>Methods: </bold>Patient samples were collected longitudinally during the acute, convalescence and recovery phases of ZIKV infection over 6 months during the Singapore outbreak in late 2016. Plasma immune mediators were profiled via multiplex microbead assay, while changes in blood cell numbers were determined with immunophenotyping.<bold>Results: </bold>Data showed the involvement of various immune mediators during acute ZIKV infection accompanied by a general reduction in blood cell numbers for all immune subsets except CD14+ monocytes. Importantly, viremic patients experiencing moderate symptoms had significantly higher quantities of interferon γ-induced protein 10, monocyte chemotactic protein 1, interleukin 1 receptor antagonist, interleukin 8, and placental growth factor 1, accompanied by reduced numbers of peripheral CD8+ T cells, CD4+ T cells, and double-negative T cells. Levels of T-cell associated mediators, including interferon γ-induced protein 10, interferon γ, and interleukin 10, were high in recovery phases of ZIKV infection, suggesting a functional role for T cells. The identification of different markers at specific disease phases emphasizes the dynamics of a balanced cytokine environment in disease progression.<bold>Conclusions: </bold>This is the first comprehensive study that highlights specific cellular changes and immune signatures during ZIKV disease progression, and it provides valuable insights into ZIKV immunopathogenesis.
- Subjects
ZIKA virus infections; METABOLIC manifestations of general diseases; CYTOKINE genetics; IMMUNOPHENOTYPING; VIREMIA; EQUIPMENT &; supplies; DIAGNOSIS; THERAPEUTICS
- Publication
Journal of Infectious Diseases, 2018, Vol 218, Issue 5, p814
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiy225