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- Title
Aortic cross-clamping influences regional net release and uptake rates of tissue-type plasminogen activator in pigs.
- Authors
SEEMAN-LODDING, H.; HAGGMARK, S.; JERN, C.; TERN, S.; JOHANSSON, G.; WINSO, O.; BIBER, B.; Häggmark, S; Jern, S; Winsö, O
- Abstract
<bold>Background: </bold>The key regulator of intravascular fibrinolysis, tissue-type plasminogen activator (t-PA), is released from a dynamic endothelial storage pool. The aim of the study was to investigate regional t-PA net release and uptake rates in response to infra-renal aortic cross-clamping (AXC) and declamping (DC).<bold>Methods: </bold>Anesthetized pigs were studied during 5 min of AXC, followed by a 35-min declamping (DC) period. Arterio-venous concentration gradients of total and active t-PA, as well as respective plasma flows, were simultaneously obtained across the preportal, hepatic, coronary and pulmonary vascular beds. Plasma levels of total t-PA (ELISA with purified porcine t-PA as standard), and active t-PA (spectrophotometric functional assay) were determined.<bold>Results: </bold>Prior to AXC, we found a high net release rate of total t-PA across the preportal vascular bed (1700 ng.min-1 P < 0.001), and a high hepatic net uptake (4900 ng.min-1, P < 0.001), while coronary and pulmonary t-PA net fluxes were small and variable. AXC per se did not induce significant alterations in net fluxes of t-PA. Following DC, preportal and coronary net releases of total t-PA increased (to 2900 ng.min-1 and 60 ng.min-1, respectively). Despite an increase in hepatic net uptake of total t-PA (to 6100 ng.min-1) after DC, a significant increase in hepatic venous total t-PA occurred.<bold>Conclusions: </bold>The release and uptake of t-PA is indicated to be dynamic and organ-specific. DC induces an acute profibrinolytic reaction in preportal organs. The high hepatic t-PA uptake capacity restricts preportal profibrinolytic events to affect the systemic circulation.
- Publication
Acta Anaesthesiologica Scandinavica, 1997, Vol 41, Issue 9, p1114
- ISSN
0001-5172
- Publication type
journal article
- DOI
10.1111/j.1399-6576.1997.tb04853.x