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- Title
Functional Validation of the RQR8 Suicide /Marker Gene in CD19 CAR-T Cells and CLL1CAR-T Cells.
- Authors
Xiong, Xia; Yu, Yibing; Jin, Xin; Xie, Danni; Sun, Rui; Lu, Wenyi; Wei, Yunxiong; Guo, Ruiting; Zhao, Mingfeng
- Abstract
Chimeric antigen receptor T cell therapy (CAR-T) is a novel treatment that has produced unprecedented clinical effects in patients with hematological malignancies. Acute adverse events often occur following adoptive immunotherapy. Therefore, a suicide gene is helpful, which is a genetically encoded mechanism that allows selective destruction of adoptively transferred T cells in the face of unacceptable toxicity. RQR8 is a gene that integrates CD34 and CD20 epitopes. In our study, we incorporated the suicide gene RQR8 into CAR-T cells, so it enabled rituximab to eliminate vector/transgene-expressing T cells via antibody-dependent cell-mediated cytotoxicity and complement dependent cytotoxicity. In this work, we explored the functionality of RQR8 CAR-T cells in vitro and in vivo. We believe that RQR8 as a safety switch will make CAR-T cell therapy safer and less costly.
- Subjects
CD19 antigen; ANTIBODY-dependent cell cytotoxicity; CHIMERIC antigen receptors; T cells
- Publication
Annals of Hematology, 2023, Vol 102, Issue 6, p1523
- ISSN
0939-5555
- Publication type
Article
- DOI
10.1007/s00277-023-05227-0