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- Title
间接竞争酶联免疫吸附测定法快速筛查 药酒中双氯芬酸.
- Authors
杨兴东; 曲 扬; 王趁趁; 毋丽华; 何贝贝; 王小慧; 胡骁飞
- Abstract
Objective To establish an indirect competitive enzyme-linked immunosorbent assay for rapid screening diclofenac (DCF) in medicinal liquor. Methods DCF was coupled to carrier protein using the active ester method and the mixed anhydride method to obtain DCF immunogen and detection antigen. BALB/c mice were immunized with DCF-bovine serum albumin, and then anti-DCF monoclonal antibody (mAb) was prepared by hybridoma technology. An indirect competitive ELISA method was established based on DCF mAb, and the performance (accuracy, precision and specificity) of the detection method was evaluated. Results Ultraviolet scanning results showed that DCF was successfully coupled to the carrier protein; the optimal hybridoma cell line (4B9) against DCF was obtained, with an IC50 value of 0.61 ng/mL; the average recovery rate of DCF added in medicinal liquor by this detection method was 85.9%, and the coefficient of variation (CV) for intra-assay (5.3%-9.7%) was greater than the CV for inter-assay (4.9%-9.1%), and there was no cross-reaction with analogues (ketoprofen, aspirin, indomethacin, ibuprofen, sulindac, naproxen, and rofecoxib). Conclusion The indirect competitive ELISA method established in this study provides a new screening method for DCF residues in medicinal liquor.
- Publication
Journal of Food Safety & Quality, 2024, Vol 15, Issue 16, p39
- ISSN
2095-0381
- Publication type
Article
- DOI
10.19812/j.cnki.jfsq11-5956/ts.20240731005