We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Development of a Ferritin-Based Nanoparticle Vaccine against Classical Swine Fever.
- Authors
Song, Yiwan; Yuan, Zhongmao; Ji, Junzhi; Ruan, Yang; Li, Xiaowen; Wang, Lianxiang; Zeng, Weijun; Wu, Keke; Hu, Wenshuo; Yi, Lin; Ding, Hongxing; Zhao, Mingqiu; Fan, Shuangqi; Li, Zhaoyao; Chen, Jinding
- Abstract
The occurrence of classical swine fever (CSF) poses a significant threat to the global swine industry. Developing an effective and safe vaccine is crucial for preventing and controlling CSF. Here, we constructed self-assembled ferritin nanoparticles fused with the classical swine fever virus (CSFV) E2 protein and a derived B cell epitope (Fe-E2B) using a baculovirus expression system (BVES), demonstrating enhanced immunogenicity. Furthermore, we provide a detailed evaluation of the immunological efficacy of the FeE2B in rabbits. The results showed that robust and sustained antibody responses were detected in rabbits immunized with the Fe-E2B nanoparticle vaccine, comparable to those elicited by commercially available vaccines. Additionally, we demonstrated that the vaccine effectively activated crucial immune factors IFN-γ and IL-4 in vivo, increasing their levels by 1.41-fold and 1.39-fold, respectively. Immunization with Fe-E2B enabled rabbits to avoid viremia and stereotypic fever after CSFV challenge. In conclusion, this study highlights the potential of ferritin nanoparticles as antigen-presenting carriers to induce robust immune responses, proposing a candidate vaccine strategy for the prevention and control of CSF.
- Subjects
CLASSICAL swine fever virus; CLASSICAL swine fever; VACCINE effectiveness; SWINE industry; B cells
- Publication
Vaccines, 2024, Vol 12, Issue 8, p948
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines12080948