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- Title
Effects of Castanopsis echinocarpa on Sensorineural Hearing Loss via Neuronal Gene Regulation.
- Authors
Rodriguez, Isabel; Nam, Youn Hee; Shin, Sung Woo; Seo, Gyeong Jin; Kim, Na Woo; Nuankaew, Wanlapa; Kim, Do Hoon; Park, Yu Hwa; Lee, Hwa Yeon; Peng, Xi Hui; Hong, Bin Na; Kang, Tong Ho
- Abstract
Sensorineural hearing loss (SNHL), characterized by damage to the inner ear or auditory nerve, is a prevalent auditory disorder. This study explores the potential of Castanopsis echinocarpa (CAE) as a therapeutic agent for SNHL. In vivo experiments were conducted using zebrafish and mouse models. Zebrafish with neomycin-induced ototoxicity were treated with CAE, resulting in otic hair cell protection with an EC50 of 0.49 µg/mL and a therapeutic index of 1020. CAE treatment improved auditory function and protected cochlear sensory cells in a mouse model after noise-induced hearing loss (NIHL). RNA sequencing of NIHL mouse cochleae revealed that CAE up-regulates genes involved in neurotransmitter synthesis, secretion, transport, and neuronal survival. Real-time qPCR validation showed that NIHL decreased the mRNA expression of genes related to neuronal function, such as Gabra1, Gad1, Slc32a1, CaMK2b, CaMKIV, and Slc17a7, while the CAE treatment significantly elevated these levels. In conclusion, our findings provide strong evidence that CAE protects against hearing loss by promoting sensory cell protection and enhancing the expression of genes critical for neuronal function and survival.
- Subjects
RESEARCH funding; NOISE-induced deafness; SENSORINEURAL hearing loss; CELL physiology; IN vivo studies; FISHES; REVERSE transcriptase polymerase chain reaction; GENE expression; MICE; ACOUSTIC nerve; MEDICINAL plants; DRUG efficacy; ANIMAL experimentation; DRUGS; OTOTOXICITY; NEUROTRANSMITTERS; EVALUATION
- Publication
Nutrients, 2024, Vol 16, Issue 16, p2716
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu16162716