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- Title
The analytical method to identify the nitrogen source for nitric oxide synthesis.
- Authors
Nasuno, Ryo; Yoshikawa, Yuki; Takagi, Hiroshi
- Abstract
Nitric oxide (NO) is a ubiquitous signaling molecule synthesized from various nitrogen sources. An analytical method to identify a nitrogen source for NO generation was developed using liquid chromatography with tandem mass spectrometry in combination with stable isotope labeling. Our method successfully detected the 15N-labeled NO-containing compound generated from 15N-labeled substrate nitrite in vitro and in vivo.
- Subjects
LIQUID chromatography-mass spectrometry; NITRIC oxide; NITROGEN compounds
- Publication
Bioscience, Biotechnology & Biochemistry, 2021, Vol 85, Issue 2, p211
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbaa046