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- Title
Avenues to Characterize the Interactions of Extended N‐Glycans with Proteins by NMR Spectroscopy: The Influenza Hemagglutinin Case.
- Authors
Fernández de Toro, Beatriz; Peng, Wenjie; Thompson, Andrew J.; Domínguez, Gema; Cañada, F. Javier; Pérez‐Castells, Javier; Paulson, James C.; Jiménez‐Barbero, Jesús; Canales, Ángeles
- Abstract
Long‐chain multiantenna N‐glycans are extremely complex molecules. Their inherent flexibility and the presence of repetitions of monosaccharide units in similar chemical environments hamper their full characterization by X‐ray diffraction or standard NMR methods. Herein, the successful conformational and interaction analysis of a sialylated tetradecasaccharide N‐glycan presenting two LacNAc repetitions at each arm is presented. This glycan has been identified as the receptor of the hemagglutinin protein of pathogenic influenza viruses. To accomplish this study, a N‐glycan conjugated with a lanthanide binding tag has been synthesized, enabling analysis of the system by paramagnetic NMR. Under paramagnetic conditions, the NMR signals of each sugar unit in the glycan have been determined. Furthermore, a detailed binding epitope of the tetradecasaccharide N‐glycan in the presence of HK/68 hemagglutinin is described.
- Subjects
GLYCANS; PROTEIN analysis; NUCLEAR magnetic resonance spectroscopy; HEMAGGLUTININ; X-ray diffraction
- Publication
Angewandte Chemie, 2018, Vol 130, Issue 46, p15271
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201807162