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- Title
Construction of an industrial polyploid strain of Saccharomyces cerevisiae containing Saprolegnia ferax β-amylase gene and secreting β-amylase.
- Authors
Choi, Eun-Young; Park, Jeong-Nam; Kim, Hee-Ok; Shin, Dong-Jun; Chun, Young-Hyun; Im, Suhn-Young; Chun, Soon-Bai; Bai, Suk
- Abstract
An industrial polyploid strain of Saccharomyces cerevisiae containing Saprolegnia ferax β-amylase gene was developed by using two yeast integrating plasmids. One plasmid was constructed that contains the geneticin resistance gene (Gtr) as the selection marker and the ribosomal DNA (rDNA) portion that comprises the 18S rDNA as the recombination site. The other plasmid contains the aureobasidin A resistance gene (AUR1-C) as the selection marker and the chromosomal Ty δ sequence as the recombination site. The β-amylase activity of one clone of Saccharomyces cerevisiae transformed sequentially with these two plasmids was approx. 9 times higher than that of Saprolegnia ferax. This type of integration was mitotically stable even after 100 generations of cell multiplication under non-selective conditions.
- Subjects
SACCHAROMYCES cerevisiae; PLASMIDS; YEAST; DNA; GENES; SAPROLEGNIA
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 21, p1785
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1020613306127