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- Title
Formulation of a lactose-free, low-cost culture medium for the production of β- D-galactosidase by Kluyveromyces marxianus.
- Authors
Furlan, Sandra A.; Schneider, Andrea L.S.; Merkle, Roselene; de Fátima Carvalho-Jonas, Maria; Jonas, Rainer
- Abstract
A lactose-free, low-cost culture medium for the production of β-d-galactosidase by Kluyveromyces marxianus was formulated. At high aeration rates (2.2 vvm) and concentrations of 100 g sugar cane molasses l-1 as carbon source and 100 g corn steep liquor l-1 as vitamin and nitrogen source an enzyme production of 708 U l-1 h was achieved. This was 20% higher than using a medium that contained lactose which is considered the primary inductor of β-d-galactosidase synthesis.
- Subjects
KLUYVEROMYCES marxianus; LACTOSE; CELL culture; SACCHAROMYCETACEAE; INDUSTRIAL microbiology; MOLASSES
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 7, p589
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005629127532