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- Title
Cross-reactivity among sapovirus recombinant capsid proteins.
- Authors
Hansman, G. S.; Natori, K.; Oka, T.; Ogawa, S.; Tanaka, K.; Nagata, N.; Ushijima, H.; Takeda, N.; Katayama, K.
- Abstract
Sapovirus (SaV), a member of the genusSapovirusin the familyCaliciviridae, is an agent of human and porcine gastroenteritis. SaV strains are divided into five genogroups (GI-GV) based on their capsid (VP1) sequences. Human SaV strains are noncultivable, but expression of the recombinant capsid protein (rVP1) in a baculovirus expression system results in the self-assembly of virus-like particles (VLPs) that are morphologically similar to native SaV. In this study, rVP1 constructs of SaV GI, GII, and GV strains were expressed in a baculovirus expression system. The structures of the GI, GII, and GV VLPs, with diameters of 41-48?nm, were morphologically similar to those of native SaV. However a fraction of GV VLPs were smaller, with diameters of 26-31?nm and spikes on the outline. This is the first report of GII and GV VLP formation and the first identification of small VLPs. To examine the cross-reactivities among GI, GII, and GV rVP1, hyperimmune rabbit antisera were raised againstEscherichia coli-expressed GI, GII, and GV N- and C-terminal VP1. Western blotting showed the GI antisera cross-reacted with GV rVP1 but not GII rVP1; GII antisera cross-reacted weakly with GI rVP1 but did not cross-react with GV rVP1; and GV antisera reacted only with GV rVP1. Also, hyperimmune rabbit and guinea pig antisera raised against purified GI VLPs were used to examine the cross-reactivities among GI, GII, and GV VLPs by an antigen enzyme-linked immunosorbent assay (ELISA). The ELISA showed that the GI VLPs were antigenically distinct from GII and GV VLPs.
- Subjects
CALICIVIRUSES; IMMUNE serums; ENZYME-linked immunosorbent assay; PROTEINS; ESCHERICHIA coli; GUINEA pigs; GASTROENTERITIS
- Publication
Archives of Virology, 2005, Vol 150, Issue 1, p21
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-004-0406-8