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- Title
HDAC2 sumo-E3 连接酶活性在DLD1 细胞迁移和增殖中的作用.
- Authors
马琳琳; 李玲; 荆昭; 李莹莹; 郭韡; 胡雪停; 邢伟; 徐祥
- Abstract
Objective: To investigate the effect of HDAC2 sumo-E3 ligase segment on proliferation and migration of DLD1 cells and the potential mechanisms. Methods: The coding sequence of the sumo-E3 ligase segment of HDAC2 (325-488aa) was chemically synthesized and ligated into a recombinant lentiviral vector. The lentiviral vector was packaged into lentiviral particles and used to infect HDAC2-knockout DLD1 cells (DLD1-HDAC2-/-), and stably transfected cells were selected and named DLDlh325-488. Then, proliferation and migration of the DLDlh325-48S cells were determined by RTCA and transwell assay: and changes in expression of proliferation- and migration-related genes were determined by qRT-PCR and immune-blotting. Results: Stable DLDlh325-488 cell line was successfully obtained. Compared to the control cells, and DLDlh325-488 cells stably expressing HDAC2 sumo-E3 ligase mutant. HDAC2 (325-488). DLDlh325-488 cells showed no significant changes in proliferation, but significantly increased migration, which was accompanied by increase expression of the migration-related MMP14. Conclusion: HDAC2 sumo-E3 ligase segment may promote the migration of DLD1 cells by upregulatingMMPl 4 expression.
- Publication
Progress in Modern Biomedicine, 2018, Vol 18, Issue 10, p1854
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2018.10.010