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- Title
Rice Pentatricopeptide Repeat Protein RF5 Restores Fertility in Hong-Lian Cytoplasmic Male-Sterile Lines via a Complex with the Glycine-Rich Protein GRP162.
- Authors
Hu, Jun; Wang, Kun; Huang, Wenchao; Liu, Gai; Gao, Ya; Wang, Jianming; Huang, Qi; Ji, Yanxiao; Qin, Xiaojian; Wan, Lei; Zhu, Renshan; Li, Shaoqing; Yang, Daichang; Zhu, Yingguo
- Abstract
The cytoplasmic male sterility (CMS) phenotype in plants can be reversed by the action of nuclear-encoded fertility restorer (Rf) genes. The molecular mechanism involved in Rf gene–mediated processing of CMS-associated transcripts is unclear, as are the identities of other proteins that may be involved in the CMS– Rf interaction. In this study, we cloned the restorer gene Rf5 for Hong-Lian CMS in rice and studied its fertility restoration mechanism with respect to the processing of the CMS-associated transcript atp6-orfH79. RF5, a pentatricopeptide repeat (PPR) protein, was unable to bind to this CMS-associated transcript; however, a partner protein of RF5 (GRP162, a Gly-rich protein encoding 162 amino acids) was identified to bind to atp6-orfH79. GRP162 was found to physically interact with RF5 and to bind to atp6-orfH79 via an RNA recognition motif. Furthermore, we found that RF5 and GRP162 are both components of a restoration of fertility complex (RFC) that is 400 to 500 kD in size and can cleave CMS-associated transcripts in vitro. Evidence that a PPR protein interacts directly with a Gly-rich protein to form a subunit of the RFC provides a new perspective on the molecular mechanisms underlying fertility restoration.
- Subjects
HYBRID rice; CYTOPLASMIC male sterility; FERTILITY; RICE; PROTEINS
- Publication
Plant Cell, 2012, Vol 24, Issue 1, p109
- ISSN
1040-4651
- Publication type
Article
- DOI
10.1105/tpc.111.093211