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- Title
PEG-Hemoglobin as a Resuscitation Solution in the Treatment of Hypovolemic Shock in the Anesthetized Rat.
- Authors
Conover, Charles D.; Linberg, Rita; Lejeune, Laura; Nagy, Melissa; Shum, Kwok L.
- Abstract
Abstract: This study was designed to determine the advantages of using the hemoglobin-based oxygen carrier, polyethylene glycol conjugated bovine hemoglobin (PEG-Hb), as an additive to Ringer's lactate solution (RLS) for the treatment of acute hemorrhage in anesthetized female rats. Different compositions of PEG-Hb and RLS were administered intravenously in a paradigm that provided 30 ml/kg of resuscitation fluid following an episode of 15 min of hypotension. Hypotension was achieved by the removal of blood (1 ml/min) from the femoral vein until the mean arterial pressure was lowered to or below 50 mmHg and subsequently maintained until resuscitation. Short-term cardiovascular assessment showed that resuscitation fluids containing PEG-Hb resulted in higher mean arterial pressure, aortic blood flow, renal blood flow, and less dramatic shifts in arterial base excess and respiratory blood gases than plain RLS. The long-term survival experiment showed lower lactate dehydrogenase, alkaline phosphatase, and serum glutamic pyruvic transaminase levels in most groups resuscitated with solutions containing PEG-Hb, but no differences in survival (100%) were observed. The data suggest that the addition of PEG-Hb to RLS improves its resuscitative effects. Specifically, a solution of 50% RLS:50% PEG-Hb appeared to have the most favorable cardiovascular and metabolic effects in this anesthetized rat hypovolemic shock resuscitation model. Presumably, the improved effects seen with the addition of PEG-Hb were due to its innate plasma expansion and oxygen-delivery capabilities.
- Subjects
POLYETHYLENE glycol; HEMOGLOBINS; HYPOVOLEMIC anemia; BLOOD flow; HYPOTENSION; THERAPEUTICS
- Publication
Artificial Organs, 1999, Vol 23, Issue 12, p1088
- ISSN
0160-564X
- Publication type
Article
- DOI
10.1111/j.1525-1594.1999.06429.x