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- Title
Effective Treatment of Patients Experiencing Primary, Acute HIV Infection Decreases Exhausted/Activated CD4+ T Cells and CD8+ T Memory Stem Cells.
- Authors
Lo Tartaro, Domenico; Camiro-Zúñiga, Antonio; Nasi, Milena; De Biasi, Sara; Najera-Avila, Marco A.; Jaramillo-Jante, Maria Del Rocio; Gibellini, Lara; Pinti, Marcello; Neroni, Anita; Mussini, Cristina; Soto-Ramírez, Luis E.; Calva, Juan J.; Belaunzarán-Zamudio, Francisco; Crabtree-Ramirez, Brenda; Hernández-Leon, Christian; Mosqueda-Gómez, Juan L.; Navarro-Álvarez, Samuel; Perez-Patrigeon, Santiago; Cossarizza, Andrea
- Abstract
Several studies have identified main changes in T- and B-lymphocyte subsets during chronic HIV infection, but few data exist on how these subsets behave during the initial phase of HIV infection. We enrolled 22 HIV-infected patients during the acute stage of infection before the initiation of antiretroviral therapy (ART). Patients had blood samples drawn previous to ART initiation (T0), and at 2 (T1) and 12 (T2) months after ART initiation. We quantified cellular HIV-DNA content in sorted naïve and effector memory CD4 T cells and identified the main subsets of T- and B-lymphocytes using an 18-parameter flow cytometry panel. We identified correlations between the patients' clinical and immunological data using PCA. Effective HIV treatment reduces integrated HIV DNA in effector memory T cells after 12 months (T2) of ART. The main changes in CD4+ T cells occurred at T2, with a reduction of activated memory, cytolytic and activated/exhausted stem cell memory T (TSCM) cells. Changes were present among CD8+ T cells since T1, with a reduction of several activated subsets, including activated/exhausted TSCM. At T2 a reduction of plasmablasts and exhausted B cells was also observed. A negative correlation was found between the total CD4+ T-cell count and IgM-negative plasmablasts. In patients initiating ART immediately following acute/early HIV infection, the fine analysis of T- and B-cell subsets has allowed us to identify and follow main modifications due to effective treatment, and to identify significant changes in CD4+ and CD8+ T memory stem cells.
- Subjects
HIV infections; B cells; IMMUNOLOGIC memory; T cells; LYMPHOCYTE subsets; CD4 antigen; PATIENTS' attitudes
- Publication
Cells (2073-4409), 2022, Vol 11, Issue 15, p2307
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells11152307