We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis.
- Authors
Molinas, Andrea; Heil, Stéphanie; Koch, Stefan
- Abstract
The CCNY gene, which encodes cyclin Y, has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Cyclin Y promotes Wnt/β-catenin signaling and autophagy, which are critical for intestinal epithelial cell (IEC) homeostasis, and may thereby contribute to wound repair in colitis. However, whether cyclin Y has an essential function in IECs is unknown. We, therefore, investigated the epithelial injury response and mucosal regeneration in mice with conditional knock-out of Ccny in the intestinal epithelium. We observed that Ccny-deficient mice did not exhibit any differences in cell proliferation and disease activity compared to wild-type littermates in the dextran sulfate sodium (DSS) colitis model. Complementary in vitro experiments showed that loss of CCNY in model IECs did not affect Wnt signaling, cell proliferation, or autophagy. Additionally, we observed that expression of the cyclin-Y-associated cyclin-dependent kinase (CDK) 14 is exceedingly low specifically in IEC. Collectively, these results suggest that cyclin Y does not contribute to intestinal epithelial homeostasis, possibly due to low levels of specific CDKs in these cells. Thus, it is unlikely that CCNY mutations are causatively involved in IBD pathogenesis.
- Subjects
INTESTINES; INFLAMMATORY bowel diseases; HOMEOSTASIS; WOUND healing; WNT signal transduction; DEXTRAN sulfate
- Publication
Cells (2073-4409), 2021, Vol 10, Issue 9, p2330
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells10092330