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- Title
Stereoselective biotransformation of phenylglycine nitrile by heterogeneous biocatalyst based on immobilized bacterial cells and enzyme preparation.
- Authors
Maksimova, Yu.; Gorbunova, A.; Demakov, V.
- Abstract
We studied the effect of a heterogeneous environment on the stereoselectivity of transformation of racemic phenylglycine nitrile. Immobilized biocatalysts were prepared by adhesion of Pseudomonas fluorescens C2 cells on carbon-containing supports and covalent crosslinking of nitrile hydratase and amidase of Rhodococcus rhodochrous 4-1 to activated chitosan as well as by the method of cross-linked aggregates. At a reaction duration of 20 h, the ratio of phenylglycine stereoisomers changes depending on the presence of support in medium. The highest optical purity of the product (enantiomeric excess of L-phenylglycine solution, 98%) is achieved when enzyme aggregates of nitrile hydratase and amidase cross-linked with 0.1% glutaraldehyde are used as a biocatalyst.
- Subjects
STEREOSELECTIVE reactions; BIOTRANSFORMATION (Metabolism); PHENYLGLYCINE; BACTERIAL cell analysis; ENZYMES; CHEMICAL synthesis
- Publication
Doklady Biochemistry & Biophysics, 2017, Vol 474, Issue 1, p183
- ISSN
1607-6729
- Publication type
Article
- DOI
10.1134/S1607672917030139