We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Identification of CENP-V as a novel microtubule-associating molecule that activates Src family kinases through SH3 domain interaction.
- Authors
Honda, Zen-ichiro; Suzuki, Takeshi; Honda, Hiroaki
- Abstract
The activation mechanisms of Src family kinases (SFKs) involve the dissociation of the intramolecular interaction between the Src homology (SH) 3 and kinase domain. This process is mediated by the intermolecular attack of outer ligands to the SH3 domain. By using a yeast two-hybrid screen, we isolated a relevant ligand involved in the activation mechanisms of SFKs. This molecule was found to be identical to a recently recognized kinetochore protein – designated as centromere protein (CENP)-V – which is required for the progression of mitosis. We show here that human CENP-V plays further roles in cell dynamics; the proline-rich region of human CENP-V associates with the SH3 domains of SFKs and potently activates SFKs, whereas another domain of CENP-V that possesses a highly conserved cysteine array confers the ability to associate with stabilized microtubules (MTs). Human CENP-V distributes to the cell protrusion and to the leading edge of migrating cells in response to external stimuli, and depletion of CENP-V by RNA interference significantly attenuates closure of a scratch wound. These findings indicate that human CENP-V is involved in directional cell motility as well as in the progression of mitosis, as a scaffolding molecule that links MTs and SFKs.
- Subjects
PROTEIN kinases; TUBULINS; KARYOKINESIS; CELL division; LEAVENING agents
- Publication
Genes to Cells, 2009, Vol 14, Issue 12, p1383
- ISSN
1356-9597
- Publication type
Article
- DOI
10.1111/j.1365-2443.2009.01355.x