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- Title
Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in preventing cell death.
- Authors
Lee, Jeong-Hwa; Takahashi, Takeshi; Yasuhara, Noriko; Inazawa, Joji; Kamada, Shinji; Tsujimoto, Yoshihide
- Abstract
Bcl-2 is the best characterized inhibitor of apoptosis, although the molecular basis of this action is not fully understood. Using a protein interaction cloning procedure, we identified a human gene designated as bis (mapped to chromosome 10q25) that encoded a novel Bcl-2-interacting protein. Bis protein showed no significant homology with Bcl-2 family proteins and had no prominent functional motif. Co-immunoprecipitation analysis confirmed that Bis interacted with Bcl-2 in vivo. DNA transfection experiments indicated that Bis itself exerted only weak anti-apoptotic activity, but was synergistic with Bcl-2 in preventing Bax-induced and Fas-mediated apoptosis. These results suggest that Bis is a novel modulator of cellular anti-apoptotic activity that functions through its interaction with Bcl-2.
- Subjects
CELL death; CARRIER proteins; APOPTOSIS; GENE transfection
- Publication
Oncogene, 1999, Vol 18, Issue 46, p6183
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1203043