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- Title
The 16S rRNA gene of Pasteuria penetrans provides an early diagnostic of infection of root-knot nematodes (Meloidogyne spp.).
- Authors
Rao, Uma; Mauchline, Tim H.; Davies, Keith G.
- Abstract
The molecular interactions between hosts and parasites is an active area of research, and the parasitism of root-knot nematodes, obligate parasites of plants, by the hyper-parasitic bacterium Pasteuria penetrans offers a model by which to investigate aspects of innate immunity. Using a pouch system we were able to demonstrate by PCR, infection of Meloidogyne incognita 4 days prior to any microscopic observations of parasitism. The pouch system, although not strictly axenic, offered a relatively clean, flexible approach with a greatly reduced number of contaminating microbial species than in any soil-based system, whereby the early stages of nematode infection could be manipulated and controlled.
- Subjects
RIBOSOMAL RNA genetics; ROOT-knot nematodes; INFECTION; HOST-parasite relationships; PARASITISM; PLANT parasites; NATURAL immunity; DIAGNOSIS
- Publication
Nematology, 2012, Vol 14, Issue 7, p799
- ISSN
1388-5545
- Publication type
Article
- DOI
10.1163/156854112X627318