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- Title
Novel alternatively spliced variant with a deletion of 52 BP in exon 6 of the progesterone receptor gene is observed frequently in breast cancer tissues (The sequences reported in this study have been deposited in the GenBank database (accession numbers AB085843, AB085844 andAB085845).)
- Authors
Hisashi Hisatomi; Norio Kohno; Kazuyuki Wakita; Kumi Nagao; Hiroyuki Hirata; Kazumasa Hikiji; Shoji Harada
- Abstract
The human progesterone receptor (PR) is a ligand-activated nuclear transcription factor that mediates progesterone action in target tissues. We found a novel alternatively spliced variant (ASV) of the PR mRNA in breast cancer tissues. The deleted transcript was characterized by an out-of-frame deletion of 52 bp in exon 6 (PR delta6/2 ASV). The PR delta6/2 ASV mRNA results in a partial defect in the region of the ligand-binding domain of the hormone receptor, where conserved residues are missing from the core of the protein. To clarify the clinical significance of the PR delta6/2 ASV, we investigated the expression of this ASV in noncancerous and cancerous tissues from patients with breast cancer using RT-PCR. The novel PR delta6/2 mRNA was detected in 24 of 39 (61.5%) cancerous tissues and in 3 of 39 (7.7%) noncancerous tissues from patients with breast cancer. PR delta6/2 ASV mRNA was expressed more frequently in breast cancer tissues than in noncancerous tissues (p < 0.0001), which suggests a possible relationship between the expression of PR delta6/2 and breast cancer. Our observations may provide a novel strategy for the genetic diagnosis of breast cancer. © 2003 Wiley-Liss, Inc.
- Subjects
CANCER genetics; BREAST cancer diagnosis; PROGESTERONE receptors; EXONS (Genetics); MESSENGER RNA; TRANSCRIPTION factors; BINDING sites
- Publication
International Journal of Cancer, 2003, Vol 105, Issue 2, p182
- ISSN
0020-7136
- Publication type
Article
- DOI
10.1002/ijc.11050